JNCI Journal of the National Cancer Institute
Oxford University Press
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Differential Distribution of Mouse Mammary Tumor Virus-Related Sequences in the DNA’s of Rats2
DOI 10.1093/jnci/62.5.1279, Volume: 62, Issue: 5,

Highlights

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Abstract

Radioactively labeled mouse mammary tumor virus (MuMTV) 60-70S RNA, obtained from virions grown In both murine and feline cells, was employed In molecular hybridization experiments to detect MuMTV-related sequences In the DNA’s of rats (Rattus norveglcus). With the use of relaxed conditions of hybridization and assay for RNA-DNA duplexes, all strains of laboratory rats and feral rats examined were shown to possess endogenous MuMTV-related DNA sequences In the low repetitive range. These sequences were related to approximately 20% of the MuMTV genome and exhibited a melting temperature (Tm) approximately 5° C lower than MuMTV-specific proviral sequences In murine (Mus musculus) DNA’s. Certain colonies of the F344 strain of rat (Fischer) contained animals whose DNA’s possessed additional MuMTV-related sequences. These sequences were related to the non-germ-line-transmitted, tumorassociated (TA) sequences of the highly oncogenic MuMTV (C3H). They were found In the DNA of some F344 rats and a cloned established F344 rat embryo cell line at a frequency of approximately one copy per haploid genome and exhibited a Tm 9° C lower than that of hybrid duplexes formed between radioactive MuMTV TA-sequence RNA and C3H mouse mammary tumor DNA. The DNA’s of rats, therefore, contained two sets of sequences that were related to sequences of the MuMTV genome: One set was germ-line transmitted, whereas the other set appeared to be transmitted in some rats via a non-germ line or infectious process.

Drohan and Schlom: Differential Distribution of Mouse Mammary Tumor Virus-Related Sequences in the DNA’s of Rats2

Notes

2 Supported in part by Public Health Service contract NOICP43223 from the National Cancer Institute.
https://www.researchpad.co/tools/openurl?pubtype=article&doi=10.1093/jnci/62.5.1279&title=Differential Distribution of Mouse Mammary Tumor Virus-Related Sequences in the DNA’s of Rats<a href="#FN1"><sup>2</sup></a>&author=W. Drohan,J. Schlom,&keyword=&subject=Investigations on Nonhuman Systems,