Microbial Biotechnology
John Wiley and Sons Inc.
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Pediococcus pentosaceus LI05 alleviates DSS‐induced colitis by modulating immunological profiles, the gut microbiota and short‐chain fatty acid levels in a mouse model
DOI 10.1111/1751-7915.13583, Volume: 13, Issue: 4,

Table of Contents

Highlights

Notes

Abstract

The P. pentosaceus LI05 treatment alleviated intestinal inflammation by maintaining the intestinal epithelial integrity and modulating the immunological profiles, gut microbiome and metabolite composition. Based on our findings, P. pentosaceus LI05 might be applied as potential preparation to ameliorate colitis.

Bian, Yang, Wu, Lv, Jiang, Wang, Wu, Li, Ye, Fang, Shi, Wang, Wang, Lu, Xie, Xia, and Li: Pediococcus pentosaceus LI05 alleviates DSS‐induced colitis by modulating immunological profiles, the gut microbiota and short‐chain fatty acid levels in a mouse model

Introduction

Inflammatory bowel disease (IBD), consisting of ulcerative colitis (UC) and Crohn’s disease (CD), is a group of disorders causing chronic inflammation of the gastrointestinal tract (Wlodarska et al. , 2015; Franzosa et al. , 2019). Multiple factors, such as genetics, host immunity and the intestinal microbiome, participate in the initial progression of the diseases (Liu et al. , 2019). The gut microbiota comprises a wide range of gut microbes that closely interact with the host to enhance the epithelial barrier function and regulate the host immunity and metabolism (Vemuri et al. , 2017, 2018). In addition, the composition of the intestinal microbiota in patients with IBD markedly differs from healthy people (Blander et al. , 2017; Lane et al. , 2017). Likewise, several animal experiments observed major changes in the structure of the gut microbiota in response to chemically induced colitis (Souza et al. , 2015; Llewellyn et al. , 2018). Despite the strong correlation between an altered intestinal microbiota structure and diseases, certain underlying mechanisms remain unknown and potential targets for intervention must be identified.

Probiotics are living microbes that exert a demonstrated beneficial effect on human health if administered at effective doses. Probiotics have been found to protect against colitis in both experimental models and human studies (Bibiloni et al. , 2005; Souza et al. , 2015; Ahl et al. , 2016). Genera such as Lactobacillus, Faecalibacterium and Akkermansia exert anti‐inflammatory effects on the intestinal mucosa of patients with IBD (Tamboli et al. , 2004; Sokol et al. , 2009; Bian et al. , 2019). Specifically, Faecalibacterium prausnitzii exerts significant beneficial effects on colitis by producing the short‐chain fatty acid (SCFA) butyrate (Qiu et al. , 2013; Rossi et al. , 2015). Additionally, Lactobacillus rhamnosus Gorbach‐Goldin (LGG) and Akkermansia muciniphila strengthen the epithelial barrier function and protect against epithelial inflammation (Chen et al. , 2016; Wu et al. , 2017; Mantegazza et al. , 2018).

Pediococcus pentosaceus, a member of the family Lactobacillaceae, is a species that has been shown to ameliorate inflammation (Lv et al. , 2014a). In addition, several species of P. pentosaceus have been reported to alleviate encephalopathy, acute liver failure, obesity and fatty liver (Bengmark et al. , 2011; Zhao et al. , 2012; Shi et al. , 2017). Our newly isolated probiotic P. pentosaceus LI05 has been shown to protect the host mucosa by strengthening the epithelial barrier function and regulating the host immunity and gut microbiota in mouse models of Clostridium difficile infection and CCl4‐induced cirrhosis (Shi et al. , 2017; Xu et al. , 2018). Based on these findings, P. pentosaceus LI05 may exert a beneficial and effect on preventing intestinal inflammation. However, its precise role in colitis and the potential mechanisms underlying these beneficial effects remain unknown.

In the present study, a DSS‐induced colitis model was established to explore the dynamic role of the intestinal microbiome. The combination of measurements of cytokine levels, the gut barrier function, 16S rRNA gene sequencing, and gut metabolite and SCFA levels was used to understand the protective role P. pentosaceus LI05 and identify the potential mechanisms involved.

Results

P. pentosaceus LI05 ameliorated DSS‐induced clinical features and intestinal injury

A DSS‐induced colitis model was successfully established to evaluate the effect of P. pentosaceus LI05 (LI05) on mice. Treatment with LI05 significantly reduced the weight loss (Fig. 1B), DAI score (Fig. 1C) and colon length shortening (Fig. 1D) induced by DSS consumption.

P. pentosaceus LI05 ameliorated DSS‐induced clinical symptoms and intestinal injury in mice.
Fig. 1
P. pentosaceus LI05 ameliorated DSS‐induced clinical symptoms and intestinal injury in mice.A. Schematic depicting the design of the animal experiment.B. The body weights of mice in the three groups recorded from day −7 to day 8 are plotted and presented as mean (SD; left panel). The bar chart represents the weight loss relative to the base weight on day 0 (right panel).C. The disease activity index (DAI) scores after DSS administration (left panel) and on day 8 (right panel) are shown for the three groups.D. Representative images of the colon in mice from the three groups (left panel) and the colon length on day 8 (right panel). *P < 0.05, ***P < 0.001 and ****P < 0.0001 according to unpaired t tests with Welch’s correction, Kruskal–Wallis tests and post hoc one‐way ANOVA.

Gastric mucosal barrier destruction is the initiating event of all known chemically induced colitis models. The main finding in our model was marked inflammatory cell infiltration (predominantly neutrophils) and a barely intact mucosal architecture with crypt abscesses and ulceration (Fig. 2A). However, mucosal damage was ameliorated in mice treated with LI05. A preserved mucosal architecture with minimal goblet cell loss and mild/moderate inflammatory infiltration was observed in the L5 group. In addition, the histopathology scores (P < 0.0001) and infiltration of neutrophils (Ly6G‐positive cells, P  < 0.01) were markedly decreased in the L5 group compared to the DP group (Fig. 2A).

P. pentosaceus LI05 ameliorated colon epithelial damage.
Fig. 2
P. pentosaceus LI05 ameliorated colon epithelial damage.A. Representative images of H&E staining, Ly6G immunohistochemical staining, ZO1 and Occludin immunofluorescence staining among three groups (left panel). Histopathology scores, the percentage of Ly6G‐positive cells, and colon expression of ZO1 (Tjp1) and Occludin in the three groups (right panel).B. The bar chart presents the plasma concentration of FITC–dextran, serum LBP levels and colon expression of CB1, CB2 and Claudin 4 among three groups. All data are presented as means ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001 according to the post hoc one‐way ANOVA.

As mentioned above, the administration of DSS caused severe intestinal mucosal damage, and the LI05 treatment ameliorated the symptoms. We hypothesized that LI05 might exert its protective effects by restoring the intestinal mucosal damage and enhancing the mucosal barrier function. We assessed Occludin, ZO1 and Claudin 4 expression using immunofluorescence staining and quantitative PCR to test this hypothesis. As presented in Figure 2A, colon tissue of L5 group showed increased ZO1 fluorescence intensities and stabilized mucosal integrity. The expression of tight junction proteins (Tjps) mRNA in the colon tissue was upregulated in L5 group by contrast with DP group (Occludin, P < 0.0001; ZO1/Tjp1, P < 0.05; Claudin 4, P  < 0.05; Fig. 2A and B).

Intestinal permeability was assessed by measuring FITC–dextran, LBP and cannabinoid receptor (CB) levels to further verify this hypothesis. The DP group presented increased FITC–dextran (P < 0.0001), CB1 (P < 0.0001), CB2 (P < 0.0001) and serum LBP (P  < 0.05) levels, whereas the LI05 treatment reversed this trend (Fig. 2B; P < 0.0001, P < 0.0001, P < 0.0001 and P  < 0.01, respectively, Fig. 2B).

P. pentosaceus LI05 ameliorated DSS‐induced colitis, displaying anti‐inflammatory properties

The restoration of intestinal permeability might be correlated with a decrease in the immunological response (Genser et al. , 2016). Therefore, the protective effects of P. pentosaceus LI05 on intestinal inflammation were explored by determining the serum and colon tissue cytokine levels. Consistent with the results of a previous experiment (Bian et al. , 2019), increased levels of the cytokines IL1α, TNF‐α, IL6, IL12P40 and MIP‐1A were observed in mice with DSS‐induced colitis. Notably, the administration of LI05 significantly decreased the levels of these cytokines. (P < 0.01, P < 0.05, P < 0.01, P < 0.0001 and P  < 0.0001, respectively, Fig. 3A). When compared to CP group, the expression of TNF‐α, IFN‐γ, IL6 and IL12P40 in colon tissue was markedly increased in mice with DSS administration (P < 0.01, P < 0.05, P < 0.001 and P  < 0.001; Fig. 3B). In the LI05 group, these expression levels were significantly downregulated and practically returned to the normal level (P < 0.01, P < 0.01, P < 0.05 and P < 0.001 respectively). Furthermore, the immunomodulatory cytokine IL10 levels upregulated in both the serum and colon after P. pentosaceus LI05 treatment (P < 0.05, P  < 0.01; Fig. 3A and B).

P. pentosaceus LI05 ameliorated DSS‐induced colitis by exerting anti‐inflammatory effects.
Fig. 3
P. pentosaceus LI05 ameliorated DSS‐induced colitis by exerting anti‐inflammatory effects.A. The serum levels of the cytokines IL1α, TNF‐α, IL6, IL10, IL12P40 and MIP‐1A in the three groups.B. Colon expression of TNF‐α, IFN‐γ, IL6, IL12P40 and IL10 in the three groups. All data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 according to the post hoc one‐way ANOVA.

P. pentosaceus LI05 ameliorated gut microbiome diversity upon DSS administration

We investigated the effects of P. pentosaceus LI05 intake on the gut microbiota composition using a 16S rRNA sequencing analysis to further explore the processes mediating the protective effects of P. pentosaceus LI05. Seventy‐two faecal samples were collected from the three groups (n  = 8 each) at three different time points (day −7, day 0 and day 8), and the identified OTU numbers are presented in Table 1. During the experiment, the OTU numbers decreased as the time of DSS administration increased in the DP (P < 0.001, day 8 vs. day 0; P < 0.001, day 8 vs. day −7) and L5 (P < 0.05, day 8 vs. day 0; P < 0.05, day 8 vs. day −7) groups. At the end of the experiment (day 8), markedly lower OTU numbers were obtained from the DP and L5 groups (P < 0.001 and P  < 0.001 respectively) than from the CP group after DSS exposure. However, a significant difference in OTU numbers was not observed between the DP and L5 groups. Throughout the experiment, the species diversity and community richness of the gut microbiome, as calculated by the observed species, Ace, Chao1, Shannon and Simpson indices, did not change significantly in the CP group (Fig. 4A). Additionally, the observed species, Ace and Chao1 indices exhibited marked reductions after DSS administration (day 8, DP vs. CP: P < 0.001, P P < 0.001 and P  < 0.01, respectively, Fig. 4A). No significant differences in these indices were observed between the DP group and L5 group, although the L5 group showed increasing trends. The species accumulation box plot reflects the species richness and sequencing depth of the analysis (Figure S1). Next, both weighted and unweighted UniFrac PCoAs, reflecting alterations in the species complexity and composition, respectively, were calculated and are presented in Figure 4B, Figure S2 and Table S2. Differences were not observed among the three groups at baseline (day −7, Fig. S2). After 7 days of LI05 administration, the L5 group was significantly separated from the CP group (day 0, P  = 0.012, Fig. S2). At the end of the experiment (day 8), the CP group and the DP group formed two distinct clusters (P  = 0.001, A > 0, Table S2). Likewise, the L5 group was clearly separated from the DP and CP groups (L5 vs. DP, P = 0.003, A > 0; L5 vs. CP, P  = 0.001, A > 0, Table S2), indicating that P. pentosaceus LI05 exerts an important effect on regulating the composition of the gut microbiota.

Table 1
OTU numbers of faecal microbiota among groups in different time.
GroupOTUs
Day −7Day 0Day 8
CP776.00 ± 15.17749.88 ± 18.11755.75 ± 17.66
DP767.88 ± 20.76726.63 ± 18.33607.38 ± 7.64***###^^^
L5743.88 ± 23.79727.13 ± 15.34651.75 ± 19.75*#^^^
*Compared to day −7, # compared to day 0, ^ compared to CP group. All data are given as means ± SEM. *P < 0.05; ***P < 0.001 by post hoc ANOVA one‐way statistical analysis.
P. pentosaceus LI05 ameliorated gut microbiome diversity induced by DSS administration.
Fig. 4
P. pentosaceus LI05 ameliorated gut microbiome diversity induced by DSS administration.A. Richness and diversity of the faecal microbiota in the three groups. Abbreviation: *CP group compared to the DP group. #CP group compared to the L5 group. All data are presented as means ± SEM.B. PCoA plot based on the weighted (left panel) and unweighted (right panel) UniFrac distances among the three groups (day 8). Each point represents a sample.

P. pentosaceus LI05 administration altered the gut microbiome composition at multiple levels

The taxonomic configuration of the intestinal microbiome is altered in patients with IBD (Tamboli et al. , 2004). Therefore, we compared the taxonomic abundance at multiple levels among the three groups. Following DSS administration, the relative abundance of Porphyromonadaceae, Odoribacter and Clostridium_sensu_stricto_1 was significantly increased in the DP group (DP vs. CP: P < 0.01, P < 0.05 and P < 0.001 respectively). However, the relative abundance of these taxa was decreased in the L5 group (L5 vs. DP: P < 0.01, P < 0.01 and P  < 0.01, respectively, Fig. S3). Throughout the experiment, P. pentosaceus LI05 supplementation clearly altered the intestinal microbiome structure. The relative abundance of Firmicutes (L5 vs. DP, P < 0.05), Verrucomicrobia and Verrucomicrobiaceae (L5 vs. CP, P < 0.0001 and P < 0.0001) increased in the L5 group. In addition, a reduction in the relative abundance of Bacteroidetes and Actinobacteria was observed in the L5 group (P < 0.05 and P < 0.001 respectively). Furthermore, the P. pentosaceus LI05 treatment markedly increased the abundance of Anaerofilum, Ruminiclostridium_5, Faecalibacterium (L5 vs. DP, P < 0.01, P < 0.01 and P < 0.001 respectively; L5 vs. CP, P < 0.01, P < 0.01 and P < 0.01 respectively) and Akkermansia (L5 vs. CP, P  < 0.0001, Figure S3), which are involved in SCFA production.

Next, we analysed the dynamic alterations in the abundance of specific taxa showing marked differences among the three groups. As shown in Figure 5A, the abundance of major phyla, such as Firmicutes, decreased after DSS administration, whereas the P. pentosaceus LI05 treatment distinctly increased the abundance. The abundance of the phylum Verrucomicrobia did not change in the DP and CP groups over time, but showed a significant increase after the P. pentosaceus LI05 treatment. Additionally, the abundance of Bacteroidaceae and Porphyromonadaceae increased rapidly in the DP group. However, the curve showing the increase in the abundance of Bacteroidaceae in the L5 group was flat, and the abundance of Porphyromonadaceae even showed a decreasing trend (Fig. 5B). Importantly, the abundance of the genera Akkermansia, Faecalibacterium and Ruminiclostridium_5 consistently increased as the time after P. pentosaceus LI05 supplementation increased (Fig. 5C).

Dynamic changes in the abundance of specific taxa at the phylum (A), family (B) and genus (C) levels among the three groups during the experiment. All data are presented as medians with interquartile ranges.
Fig. 5
Dynamic changes in the abundance of specific taxa at the phylum (A), family (B) and genus (C) levels among the three groups during the experiment. All data are presented as medians with interquartile ranges.

We performed the LEfSe analysis of differences between groups to further investigate the prognostic microbial markers. No significant difference was observed between three groups prior to DSS administration (Figure S4). At the end of the experiment (day 8), the DP group showed a considerably altered microbiota structure, which was mainly reflected in the enrichment of the genera Bacteroides, Escherichia_Shigella and Turicibacter (LDA score (log10 ) > 4.8, Fig. S5A) in contrast with the CP group. In contrast, the family Ruminococcaceae and genus Ruminiclostridium_9 were significantly enriched in the L5 group (LDA score (log10 ) > 4.8, Fig. S5B). Consistent with the results described above, the genus Odoribacter was enriched in the DP group, while the family Verrucomicrobiaceae and genus Akkermansia were enriched in the L5 group.

The P. pentosaceus LI05 treatment altered the DSS‐induced changes in the faecal metabolic profiles

A non‐targeted analysis of the metabolite composition was determined in the present study to link the intestinal microbiome with metabolic functions, and 330 metabolites were identified (data not shown). The total ion stacking chromatogram of QC samples and the box plot of the metabolite intensity distribution indicated the stability and repeatability of the experiment (Fig. S6). A PCA was performed to visualize the differentially altered metabolites among the groups and showed distinctly separated clusters (Fig. S7A). An OPLS‐DA was performed, as presented in Figure S7B and C. The score plot showed a marked separation of the metabolic profiles between the groups (L5 vs. DP, Q2 cum = 0.91; DP vs. CP, Q2 cum = 0.979). The metabolites that were differentially altered by P. pentosaceus LI05 administration were selected using OPLS‐DA (VIP > 1, P  < 0.05). One hundred twenty‐five selected metabolites are presented in the heat map and are mainly associated with the following pathways: amino acids, lipids, purine and carbohydrates (Figures S7D and S8). Importantly, the levels of some metabolites involved in the amino and purine pathways were significantly increased or decreased in the L5 group compared with the DP group (Fig. S7D). The levels of amino acids such as tryptophan and phenylalanine decreased after the LI05 treatment, whereas the levels of the amino acid arginine and the purine nucleoside adenosine were increased by LI05 in the present study.

P. pentosaceus LI05 increased the production of SCFAs

Significant increases in SCFA productions were observed in faecal samples from the L5 group. Six major SCFAs, acetic acid, propionic acid, isobutyric acid, butyric acid, 2‐methylbutyric acid and valeric acid, were selected and measured in the faecal samples. The acetic acid and butyric acid concentrations were remarkably decreased by DSS administration (DP vs. CP: P < 0.05 and P  < 0.001, respectively, Fig. 6). In comparison, P. pentosaceus LI05 administration noticeably increased the levels of acetic acid, propionic acid, isobutyric acid and butyric acid (L5 vs. DP: P < 0.01, P < 0.0001 and P  < 0.01, respectively, Fig. 6). However, no significant differences were observed in the production of isobutyric acid, 2‐methylbutyric acid and valeric acid.

P. pentosaceus LI05 administration increased the production of short‐chain fatty acids (SCFAs). The faecal concentration of SCFAs was determined using GC‐MS, including acetic acid, propionic acid, isobutyric acid, butyric acid, 2‐metylbutic acid and valeric acid. All data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 according to the post hoc one‐way ANOVA.
Fig. 6
P. pentosaceus LI05 administration increased the production of short‐chain fatty acids (SCFAs). The faecal concentration of SCFAs was determined using GC‐MS, including acetic acid, propionic acid, isobutyric acid, butyric acid, 2‐metylbutic acid and valeric acid. All data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 according to the post hoc one‐way ANOVA.

Correlations of the P. pentosaceus LI05‐modified gut microbiota with gut barrier markers, inflammatory cytokines and SCFAs indicate its important effect on DSS‐induced colitis

Gut microbes have been shown to exert beneficial effects on IBD (Tamboli et al. , 2004). We conducted a correlation analysis between the altered intestinal bacteria, gut barrier markers, inflammatory cytokines and SCFAs to further explore the protective effects of the P. pentosaceus LI05‐modified gut microbiota. Gut bacteria, the abundances of which were markedly increased in the DP group, might be involved in the aggravation of colitis. For example, Actinobacteria positively correlated with TNF‐α, IL1α, IL6, IL12P40, and MIP‐1A levels and intestinal permeability (P < 0.05, P < 0.001, P < 0.001, P < 0.01, P < 0.001 and P  < 0.001, respectively, Fig. 7). Similarly, Bacteroidetes and Odoribacter positively correlated with IL1α, IL12P40 and MIP‐1A levels and intestinal permeability (P < 0.01, P < 0.05, P < 0.01 and P  < 0.01, respectively, Fig. 7). In contrast, the levels of these inflammatory cytokines negatively correlated with bacteria exhibiting an increased abundance after P. pentosaceus LI05 administration. Positive correlations were identified between IL10 levels, and almost all bacteria with markedly increased abundances in the L5 group. Specifically, Verrucomicrobiaceae, Verrucomicrobia and Akkermansia displayed positive correlations with the levels of gut barrier markers, such as Tjp1. In addition, the levels of SCFAs, such as acetic acid, propionic acid and butyric acid, positively correlated with Verrucomicrobiaceae, Verrucomicrobia and Akkermansia, whereas Faecalibacterium positively correlated with butyric acid levels (Fig. 7). Based on these data, the alterations in the gut microbiota induced by P. pentosaceus LI05 treatment play essential roles in alleviating DSS‐induced colitis by maintaining the epithelial barrier function, inhibiting inflammatory responses and reducing SCFA production.

Correlations of representative microbial taxa, gut barrier markers, inflammatory cytokines and SCFAs between the DP and L5 groups. Spearman’s rank correlation coefficients were calculated. Blue indicates positive correlations, and red indicates negative correlations. Significant differences were indicated as follows: *P < 0.05, **P < 0.01 and ***P < 0.001.
Fig. 7
Correlations of representative microbial taxa, gut barrier markers, inflammatory cytokines and SCFAs between the DP and L5 groups. Spearman’s rank correlation coefficients were calculated. Blue indicates positive correlations, and red indicates negative correlations. Significant differences were indicated as follows: *P < 0.05, **P < 0.01 and ***P < 0.001.

Discussion

Gut microbes, which are relevant to host immunity and metabolism, are key factors contributing to IBD pathogenesis and development (Tamboli et al. , 2004; Sartor, 2008; Marion‐Letellier et al. , 2016). According to multiple studies, P. pentosaceus LI05 exerts an important effect on protecting the host intestine by regulating the gut microbiome, thereby strengthening the gut barrier and modulating the mucosal immunity, as well as host metabolism (Bengmark et al. , 2011; Zhao et al. , 2012; Lv et al. , 2014b; Shi et al. , 2017; Xu et al. , 2018). Therefore, our study aimed to evaluate alterations in the gut microbiota, immune responses and metabolites (SCFA production) in a mouse model of DSS‐induced colitis after treatment with P. pentosaceus LI05. Mice treated with P. pentosaceus LI05 in the present study showed a significant amelioration of symptoms, including improvements in body weight loss, DAI score, colon length shortening and intestinal permeability. In addition, marked shifts in the compositions of the gut microbiota and metabolites were observed in mouse faecal samples, and the altered taxa might mediate dysbiosis in colitis. Intriguingly, the administration of P. pentosaceus LI05 increased the abundance of specific genera, such as Akkermansia and Faecalibacterium, and helped regulate the gut microbiota, reduce host inflammation and increase SCFA production.

Epithelial injury is the initiating event in DSS‐induced colitis models, and intestinal permeability is an important metric representing the severity of colitis (Wirtz et al. , 2017). Intestinal permeability is markedly increased after DSS exposure (Png et al. , 2010). Notably, probiotics increase the thickness of colonic mucus layer by regulating bacterial products (e.g. peptides and SCFAs), resulting in a strengthened intestinal barrier (Meyer‐Hoffert et al. , 2008; Ahl et al. , 2016). Our results consistently showed decreased intestinal permeability and serum LPS levels, as well as increased expression of tight junction markers after the application of P. pentosaceus LI05.

The recovery of intestinal permeability might be related to a reduced inflammatory response (Genser et al. , 2016). Based on accumulating evidence, epithelium destruction and a severe inflammatory response occur in patients with IBD (Souza et al. , 2005; Coskun, 2014). Similarly, the damaged intestinal epithelium induced by the administration of DSS results in increased exposure of immune cells to antigens, consequently inducing a profound immune response (Souza et al. , 2015; Llewellyn et al. , 2018). In the present study, the P. pentosaceus LI05 treatment exerted protective effects on colonic inflammation by decreasing the levels of the cytokines and chemokines IL1α, IL6, TNF‐α and MIP‐1A, increasing the level of the anti‐inflammatory cytokine IL10 and reducing neutrophil infiltration in the intestine. As reported in previous studies, neutrophil infiltration is observed in rectal biopsies from patients with UC, and DSS‐induced colitis is characterized by a significant increase in neutrophil accumulation in the colon (Lampinen et al. , 2005; Vieira et al. , 2013; Ranganathan et al. , 2013). Hence, the inhibition of neutrophil infiltration in the intestine might be an essential mechanism involved in the protection provided by probiotics in patients with IBD. The levels of the proinflammatory cytokines IL1β, IL6 and TNF‐α are increased in both animal models of colitis and patients with UC (Liu et al. , 2013; Lee et al. , 2013; Tao et al. , 2013). These cytokines are involved in disrupting the tight junctions of the epithelial layer through the nuclear factor (NF)‐κB signalling pathway (Liu et al. , 2013; Woodhouse et al. , 2018). In addition, interventions that block receptors of TNF‐α and IL6 markedly attenuate the progression of IBD (Hibi et al. , 2003). Moreover, chemokines such as MIP‐1A are generated rapidly during inflammation and have been observed to be involved in monocyte/macrophage activation (Kostova et al. , 2015). Remarkably, compelling evidence has shown that the level of the anti‐inflammatory cytokine IL10 is increased after probiotic treatments (e.g. Lactobacillus GG, VSL#3, and Akkermansia) in animal models of colitis (Dieleman et al. , 2003; Lammers et al. , 2003; Bian et al. , 2019), consistent with our findings.

The gut microbiome plays critical roles in our bodies by regulating health and disease progression (Hor et al. , 2019). A distinct alteration the intestinal microbiota composition occurs in patients with IBD and mice treated with DSS (Manichanh et al. , 2006; Frank et al. , 2007; Dicksved et al. , 2012; Putignani et al. , 2016), as evidenced by decreased species richness and biodiversity. A reduced diversity has also been observed in the inflamed colon within the same patient with IBD (Sepehri et al. , 2007). Similar results were obtained in the present study, as we observed broad intestinal microbiota dysbiosis, including reduced OTU numbers and α diversity, in mice administered DSS. Although the P. pentosaceus LI05 treatment did not completely reverse the altered diversity, it increased the microbial diversity in faecal samples to some extent.

The abundance of specific bacteria, such as Enterobacteriaceae, is increased in patients suffering from IBD and in mice with chemically induced colitis (Lupp et al. , 2007). Escherichia/Shigella and Escherichia coli, which belong to the family Enterobacteriaceae, are enriched in patients with IBD (Sokol et al. , 2006; Quigley et al. , 2013). Escherichia/Shigella might increase intestinal permeability and exacerbate colitis (Quigley et al. , 2013). Consistent with these findings, Escherichia/Shigella was enriched in mice treated with DSS in our study, indicating that the inflammatory environment might be conducive to the overgrowth of this bacterial clade and the exacerbation of the intestinal inflammation.

The abundance of the phylum Firmicutes and particularly the class Clostridia is decreased in patients IBD (Frank et al. , 2007). Bacteria of the class Clostridia have previously been shown to inhibit host colon inflammation by directly regulating colonic regulatory T cells (Tregs; Atarashi et al. , 2013). Bacteria within the class Clostridia, particularly the family Ruminococcaceae and the genus Faecalibacterium, play an essential role in SCFA production (Pryde et al. , 2002). SCFAs, which are fermented from fibre, have been observed to regulate Treg induction and inhibit neutrophil activation, which can oppose colitis (Maslowski et al. , 2009; Arpaia et al. , 2013; Furusawa et al. , 2013; Smith et al. , 2013). In particular, butyrate, one of the primary energy sources for colon cells, has been proposed to exert essential protective effects on IBD and colon cancer (Wang et al. , 2018). Additionally, butyrate promotes mucin production, modulates innate immune cells and suppresses NF‐κB activation, (McHardy et al. , 2013). Faecalibacterium prausnitzii, the only identified species in the genus Faecalibacterium, has been shown to produce butyrate to improve intestinal barrier function and maintain the immune balance in previous studies (Carlsson et al. , 2013). Consistent with these findings, Faecalibacterium was significantly enriched in mice treated with P. pentosaceus LI05 in our study. Moreover, butyric acid production was markedly increased after P. pentosaceus LI05 administration and positively correlated with an increase in Faecalibacterium abundance.

Notably, an increased abundance of the genus Akkermansia was observed in mice treated with P. pentosaceus LI05 in our study. Multiple studies have reported a reduced abundance of Akkermansia in both patients with IBD and animals with colitis (Png et al. , 2010; Bian et al. , 2019). Akkermansia, a mucin‐degrading bacterium, has been shown to degrade mucin into multiple metabolites, such as SCFAs, subsequently regulating host immune responses and biological functions, including glucose and lipid metabolism (Derrien et al. , 2004). Furthermore, Akkermansia promotes mucus production, maintains the intestinal barrier integrity and thereby relieves colonic mucosal inflammation (Everard et al. , 2013). Consistent with these findings, Akkermansia negatively correlated with the levels of almost all tested proinflammatory cytokines and positively correlated with the levels of intestinal barrier markers and SCFAs (acetic acid, propionic acid and butyric acid) in the present study.

Based on the evidence from compelling studies, gut microbes modulate the gastrointestinal metabolites (Rao and Samak, 2013; Hor et al. , 2019). Faecal amino acid levels are increased in patients with IBD (Marchesi et al. , 2007; Jansson et al. , 2009) and potentially represent biomarkers for the assessment of IBD (Hisamatsu et al. , 2012). Consistent with these results, the levels of the amino acids tryptophan and phenylalanine were increased after DSS exposure and decreased by P. pentosaceus LI05 administration. Another amino acid, arginine, can be processed into immunomodulatory metabolites (Postler and Ghosh, 2017), and its biosynthesis was increased in the L5 group. In addition, a marked difference in purine metabolism was observed between the DP and L5 groups. Adenosine is a purine nucleoside, and its levels were significantly increased after P. pentosaceus LI05 treatment in the present study. These findings were consistent with previous reports showing that adenosine regulates innate lymphoid cell function and alleviates colitis (Kurtz et al. , 2014; Crittenden et al. , 2018). Thus, P. pentosaceus LI05 administration modulates the gut microbiota by increasing its similarity to a healthy gut microbiota, and the protective effects of P. pentosaceus LI05 on colitis involve the ‐modification of the gut microbiota. The P. pentosaceus LI05‐modified gut microbiota played key roles in improving gut barrier function, reducing immune responses and promoting SCFA production.

The colitis model we used does not completely represent the clinical characteristics and pathogenesis of IBD. Clinical experiments are needed to investigate the effects of P. pentosaceus LI05 on humans. Although we observed a distinct distribution of the microbiota among the groups and a strong correlation between the colitis‐related and P. pentosaceus LI05‐modified gut microbiota, other potential mechanisms might also be involved.

Overall, the P. pentosaceus LI05 treatment alleviated the intestinal inflammation by maintaining gut barrier function and modulating immunological profiles, gut microbiota and metabolite compositions. As shown in the present study, P. pentosaceus LI05 might be applied as a potential preparation to ameliorate colitis.

Experimental procedures

Preparation of P. pentosaceus LI05

P. pentosaceus LI05 (CGMCC 7049), which was isolated from the faeces of healthy individuals, was cultured anaerobically overnight at 37°C in Man Rogosa Sharpe (MRS) broth (Oxoid, Thermo Fisher Biochemicals), as previously described (Shi et al. , 2017). Bacteria were washed with sterile phosphate‐buffered saline (PBS) and re‐suspended in PBS to a density of 1.5 × 1010 CFU (colony forming units)/ml before use. Two hundred microlitres of the mixture with 3 × 109 CFU P. pentosaceus LI05 was prepared for each mouse.

Mice and experimental colitis induction

Male C57BL/6 mice were purchased from SLAC Lab (Shanghai, China) and housed under controlled conditions (specific pathogen‐free, 12 h light and dark cycle). Animals were randomly assigned to the groups CP, DP and L5 (n = 8 each). Mice in the L5 (DSS + LI05) group were treated with 200 μl of P. pentosaceus LI05 by oral gavage from day −7 to day 6 (Fig. 1A). Mice in the CP (water + PBS) and DP (DSS + PBS) groups were treated with 200 μl of sterile PBS instead of the probiotic.

Acute colitis was induced by administering 2% (wt/vol) DSS (molecular weight: 36 000–50 000; MP Biomedicals). Briefly, mice were provided with DSS (DP and L5 groups) and drinking water (CP group) ad libitum from day 0 to day 6 (Fig. 1A). A freshly prepared DSS solution was administered every 2 days throughout our experiment. Mouse weights and faecal conditions were recorded daily; mice were humanely sacrificed on day 8; and serum and colon samples were collected. Faecal samples were collected before oral gavage (day −7), before DSS administration (day 0) and after colitis induction (day 8). Distal parts of the colon segments were measured and fixed with 10% formalin for the subsequent histological analysis. The remaining colon, serum and faecal samples were collected for subsequent procedures. The present study was approved by the ethics committee of the First Affiliated Hospital of Zhejiang University (2019‐1069).

Disease activity index evaluation

A disease activity index (DAI) score was recorded daily after the initiation of DSS administration to further quantify the colitis severity. The DAI score (0–12) consists of three parts based on the clinical features: weight loss, faecal blood and consistency (Wirtz et al. , 2017). Faecal blood was assessed using a faecal occult blood reagent (Baso, BA2020E).

Analysis of intestinal permeability and histological examination

A permeability probe, fluorescein isothiocyanate‐conjugated (FITC)–dextran (3–5 kDa, Sigma‐Aldrich), was applied for the intestinal permeability analysis, as previously described (Bian et al. , 2019). Animals were treated with FITC–dextran (12 mg per 20 g body weight) by gavage after an overnight fast. The FITC–dextran concentration in plasma samples was determined 4 h after oral gavage using spectrophotofluorometry (490/525 nm).

The fixed and embedded distal colon samples were then processed for haematoxylin and eosin (H&E) staining. Randomly selected slides from each group were observed by an independent pathologist using the NanoZoomer Digital Pathology system (Hamamatsu Photonics, KK, Japan). The degree of the histopathological changes was calculated by calculating the histological activity index, which consists of inflammatory cell infiltration (1–3) and the intestinal architecture (1–3), as previously reported ( Erben et al. , 2014).

Immunofluorescence and immunohistochemical staining

Embedded colon sections were immunostained with antibodies (ZO1, Occludin and Ly6G), as previously described (Chung et al. , 2014). Images were scanned using a fluorescence confocal microscope (Zeiss, Jena, Germany). Five fields of view from each sample were selected randomly, and Image‐Pro Plus software was used to calculate the Ly6G+ cell ratio.

Measurement of serum cytokine and endotoxin concentrations

The serum concentrations of cytokines and chemokines, including interleukin (IL)1α, IL6, IL10, IL12 (p40), tumour necrosis factor alpha (TNF‐α) and macrophage inflammatory protein (MIP)‐1A, were assessed using a cytokine assay kit (Bio‐Rad, CA, USA), according to the manufacturer’s instructions.

A Guduo lipopolysaccharide binding protein (LBP) ELISA kit (Shanghai, China) was used to measure the serum endotoxin levels, as previously described (Ye et al. , 2018).

qRT‐PCR analysis

Total colonic RNA was extracted with an RNeasy Plus Mini kit (Qiagen, CA, USA) according to the manufacturer’s protocols. A PrimeScript RT reagent kit (Takara Biomedicals, Kusatsu, JPN) was used to reverse transcribe the RNA into cDNAs. Then, mRNA expression was determined in duplicate with SYBR Premix Ex Taq II reagent (Takara Biomedicals) using the ViiA7 real‐time PCR system (Applied Biosystems, Massachusetts, USA). All gene expression levels were assessed using the 2−ΔΔCT method and normalized to β‐actin expression. The primer sequences are provided in Table S1.

16S rRNA gene sequencing analysis

The 16S rRNA gene sequencing analysis was performed to determine the microbial composition, as previously reported (Bian et al. , 2019). Briefly, a QIAamp Fast DNA Stool Mini kit (Qiagen, Valencia, USA) was used to extract total DNA from faecal samples (200 ± 10 mg). The amplification of 16S rRNA gene was conducted using specific primers (e.g. 16S V4:515F‐806R). Following PCR and purification, an Ion Plus Fragment Library kit 48 rxns (Thermo Scientific) and an Ion S5™ XL platform were used to generate the sequencing libraries. Then, 400 bp/600 bp clean single‐end reads were obtained using a quality control (QC) procedure. Sequences displaying ≥ 97% similarity were considered as the same operational taxonomic units (OTUs).

QIIME (version 1.7.0) was used to analyse the α diversity, including the observed species, Chao1, Shannon, Simpson and ACE indices, as well as to perform the principal coordinate analysis (PCoA) of weighted and unweighted UniFrac distances. A linear discriminant analysis effect size (LefSe) analysis was conducted online (Bajaj 2014). The intergroup difference was tested using a multi‐response permutation procedure (MRPP). The correlations of bacterial taxa with colitis‐related indices and SCFA levels were determined by calculating Spearman’s rank correlation coefficients and are presented as heat maps with P < 0.05 generated using R software (version 2.15.3).

The 16S rRNA gene sequencing data obtained from the 72 samples in our study have been uploaded to the NCBI SRA database (PRJNA 594232).

Analysis of the metabolic profile

Faecal metabolic profiling assessments were conducted using gas chromatography–mass spectrometry (GC‐MS). An accurately weighed faecal sample (60 mg) was mixed with 40 μl of the internal standard (2‐chloro‐l‐phenylalanine dissolved in methanol, 0.3 mg ml−1) and 360 μl of cold methanol. After the sample was subjected to grinding and ultrasound‐associated extraction for 30 min, 200 μl of chloroform was added and the sample was vortexed (60 Hz, 2 min). Then, 400 μl of water was added to the sample, and the sample was extracted for 30 min before an incubation at −20°C for 30 min. The sample was centrifuged (12 000 r.p.m., 10 min, 4°C), and 300 μl of the supernatant was obtained for further analysis. QC samples were obtained by pooling small aliquots of each sample and then divided into five samples. The supernatant was dried with a vacuum dryer, and 80 μl of methoxylamine hydrochloride (dissolved in pyridine, 15 mg ml−1) was added. The mixture was vortexed and incubated at 37°C for 90 min. Then, 80 μl of BSTFA (1% TMCS), 20 μl of n‐hexane and 10 μl of the internal standard (C8/C9/C10/C12/C14/C16, 0.8 mg ml−1; C18/C20/C22/C24/C26, 0.4 mg ml−1) were added, vortexed and incubated at 70°C for 60 min. The sample was incubated at room temperature for 30 min prior to the GC‐MS analysis. Metabolites were identified using an untargeted GC‐MS database from Lumingbio. A principal component analysis (PCA) and orthogonal partial least‐squares‐discriminant analysis (OPLS‐DA) were conducted to visualize the differences and select the differentially altered metabolites among the groups; metabolites with a variable importance in the projection (VIP) value > 1 were selected as significantly different.

SCFAs in faeces (20 mg) were extracted and mixed with 500 μl of water containing 10 μg ml−1 hexanoic acid‐d3 as an internal control. Then, the samples were vortexed, centrifuged (15 000× r.p.m., 5 min) and mixed with 500 μl of ethyl acetate (5% concentrated sulfuric acid). Following a second centrifugation, the supernatant was incubated at 4°C for 30 min and transferred to specimen tubes for further analysis. Standard mixtures of six SCFAs were prepared using the same procedures used for the samples.

Statistical analysis

The data are presented as the means ± SEM or medians with interquartile ranges. The Kolmogorov–Smirnov test was used to analyse the normality of the data. For most data, the Kruskal–Wallis test (for data with non‐normal distributions) or one‐way ANOVA with Bonferroni’s post hoc test (for data with normal distributions) was used to determine the significance between three groups. Analyses of colon length, LBP, FITC–dextran, Tjp1, Occludin, Claudin 4, CB1, CB2, serum cytokine levels, α‐diversity and metabolite levels were performed using one‐way ANOVA with Bonferroni’s post hoc test. Body weight loss was analysed using an unpaired t test followed by Welch’s corrections. DAI, histopathological score and taxonomic relative abundance were assessed using the Kruskal–Wallis test. A LefSe analysis was conducted between three groups and at three time points (day −7, day 0 and day 8). Statistical analyses were conducted using R software (version 2.15.3) and SPSS (version 20.0). Images were constructed using GraphPad Prism (version 7.0) and R software (version 2.15.3). Two‐tailed P values < 0.05 were considered statistically significant.

Conflicts of interest

The authors declare no conflicts of interest.

Authors’ contributions

Bian, Yang, Wu, Li, Jiang and Fang designed and performed the experiments; Bian, Lv, Wang, Lu, Xie, Xia and Shi analysed the data; and Bian, Ye and Li wrote and reviewed the manuscript.

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https://www.researchpad.co/tools/openurl?pubtype=article&doi=10.1111/1751-7915.13583&title= <i>Pediococcus pentosaceus</i> LI05 alleviates DSS‐induced colitis by modulating immunological profiles, the gut microbiota and short‐chain fatty acid levels in a mouse model&author=Xiaoyuan Bian,Liya Yang,Wenrui Wu,Longxian Lv,Xianwan Jiang,Qing Wang,Jingjing Wu,Yating Li,Jianzhong Ye,Daiqiong Fang,Ding Shi,Kaicen Wang,Qiangqiang Wang,Yanmeng Lu,Jiaojiao Xie,Jiafeng Xia,Lanjuan Li,&keyword=&subject=Research Article,Research Articles,